Combinatorial modification of multiple lignin traits in trees through multigene cotransformation.
Identifieur interne : 004475 ( Main/Exploration ); précédent : 004474; suivant : 004476Combinatorial modification of multiple lignin traits in trees through multigene cotransformation.
Auteurs : Laigeng Li [États-Unis] ; Yihua Zhou ; Xiaofei Cheng ; Jiayan Sun ; Jane M. Marita ; John Ralph ; Vincent L. ChiangSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2003.
Descripteurs français
- KwdFr :
- ADN des plantes (génétique), Coenzyme A ligases (génétique), Cytochrome P-450 enzyme system (génétique), Gènes de plante (MeSH), Immunohistochimie (MeSH), Lignine (composition chimique), Lignine (métabolisme), Mixed function oxygenases (génétique), Modèles biologiques (MeSH), Populus (génétique), Populus (métabolisme), Protéines végétales (MeSH), Rhizobium (génétique), Spectroscopie par résonance magnétique (MeSH), Séquence nucléotidique (MeSH), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- composition chimique : Lignine.
- génétique : ADN des plantes, Coenzyme A ligases, Cytochrome P-450 enzyme system, Mixed function oxygenases, Populus, Rhizobium.
- métabolisme : Lignine, Populus.
- Gènes de plante, Immunohistochimie, Modèles biologiques, Protéines végétales, Spectroscopie par résonance magnétique, Séquence nucléotidique, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Coenzyme A Ligases (genetics), Cytochrome P-450 Enzyme System (genetics), DNA, Plant (genetics), Genes, Plant (MeSH), Immunohistochemistry (MeSH), Lignin (chemistry), Lignin (metabolism), Magnetic Resonance Spectroscopy (MeSH), Mixed Function Oxygenases (genetics), Models, Biological (MeSH), Plant Proteins (MeSH), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (metabolism), Rhizobium (genetics), Transformation, Genetic (MeSH).
- MESH :
- chemical , chemistry : Lignin.
- chemical , genetics : Coenzyme A Ligases, Cytochrome P-450 Enzyme System, DNA, Plant, Mixed Function Oxygenases.
- chemical , metabolism : Lignin.
- genetics : Populus, Rhizobium.
- metabolism : Populus.
- Base Sequence, Genes, Plant, Immunohistochemistry, Magnetic Resonance Spectroscopy, Models, Biological, Plant Proteins, Plants, Genetically Modified, Transformation, Genetic.
Abstract
Lignin quantity and reactivity [which is associated with its syringyl/guaiacyl (S/G) constituent ratio] are two major barriers to wood-pulp production. To verify our contention that these traits are regulated by distinct monolignol biosynthesis genes, encoding 4-coumarate-CoA ligase (4CL) and coniferaldehyde 5-hydroxylase (CAld5H), we used Agrobacterium to cotransfer antisense 4CL and sense CAld5H genes into aspen (Populus tremuloides). Trees expressing each one and both of the transgenes were produced with high efficiency. Lignin reduction by as much as 40% with 14% cellulose augmentation was achieved in antisense 4CL plants; S/G-ratio increases as much as 3-fold were observed without lignin quantity change in sense CAld5H plants. Consistent with our contention, these effects were independent but additive, with plants expressing both transgenes having up to 52% less lignin, a 64% higher S/G ratio, and 30% more cellulose. An S/G-ratio increase also accelerated cell maturation in stem secondary xylem, pointing to a role for syringyl lignin moieties in coordinating xylem secondary wall biosynthesis. The results suggest that this multigene cotransfer system should be broadly useful for plant genetic engineering and functional genomics.
DOI: 10.1073/pnas.0831166100
PubMed: 12668766
PubMed Central: PMC153659
Affiliations:
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Marita</name></author><author><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name></author><author><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Coenzyme A Ligases (genetics)</term><term>Cytochrome P-450 Enzyme System (genetics)</term><term>DNA, Plant (genetics)</term><term>Genes, Plant (MeSH)</term><term>Immunohistochemistry (MeSH)</term><term>Lignin (chemistry)</term><term>Lignin (metabolism)</term><term>Magnetic Resonance Spectroscopy (MeSH)</term><term>Mixed Function Oxygenases (genetics)</term><term>Models, Biological (MeSH)</term><term>Plant Proteins (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Rhizobium (genetics)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (génétique)</term><term>Coenzyme A ligases (génétique)</term><term>Cytochrome P-450 enzyme system (génétique)</term><term>Gènes de plante (MeSH)</term><term>Immunohistochimie (MeSH)</term><term>Lignine (composition chimique)</term><term>Lignine (métabolisme)</term><term>Mixed function oxygenases (génétique)</term><term>Modèles biologiques (MeSH)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (MeSH)</term><term>Rhizobium (génétique)</term><term>Spectroscopie par résonance magnétique (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Coenzyme A Ligases</term><term>Cytochrome P-450 Enzyme System</term><term>DNA, Plant</term><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des plantes</term><term>Coenzyme A ligases</term><term>Cytochrome P-450 enzyme system</term><term>Mixed function oxygenases</term><term>Populus</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lignine</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Genes, Plant</term><term>Immunohistochemistry</term><term>Magnetic Resonance Spectroscopy</term><term>Models, Biological</term><term>Plant Proteins</term><term>Plants, Genetically Modified</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes de plante</term><term>Immunohistochimie</term><term>Modèles biologiques</term><term>Protéines végétales</term><term>Spectroscopie par résonance magnétique</term><term>Séquence nucléotidique</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lignin quantity and reactivity [which is associated with its syringyl/guaiacyl (S/G) constituent ratio] are two major barriers to wood-pulp production. To verify our contention that these traits are regulated by distinct monolignol biosynthesis genes, encoding 4-coumarate-CoA ligase (4CL) and coniferaldehyde 5-hydroxylase (CAld5H), we used Agrobacterium to cotransfer antisense 4CL and sense CAld5H genes into aspen (Populus tremuloides). Trees expressing each one and both of the transgenes were produced with high efficiency. Lignin reduction by as much as 40% with 14% cellulose augmentation was achieved in antisense 4CL plants; S/G-ratio increases as much as 3-fold were observed without lignin quantity change in sense CAld5H plants. Consistent with our contention, these effects were independent but additive, with plants expressing both transgenes having up to 52% less lignin, a 64% higher S/G ratio, and 30% more cellulose. An S/G-ratio increase also accelerated cell maturation in stem secondary xylem, pointing to a role for syringyl lignin moieties in coordinating xylem secondary wall biosynthesis. The results suggest that this multigene cotransfer system should be broadly useful for plant genetic engineering and functional genomics.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12668766</PMID><DateCompleted><Year>2003</Year><Month>06</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0027-8424</ISSN><JournalIssue CitedMedium="Print"><Volume>100</Volume><Issue>8</Issue><PubDate><Year>2003</Year><Month>Apr</Month><Day>15</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation></Journal><ArticleTitle>Combinatorial modification of multiple lignin traits in trees through multigene cotransformation.</ArticleTitle><Pagination><MedlinePgn>4939-44</MedlinePgn></Pagination><Abstract><AbstractText>Lignin quantity and reactivity [which is associated with its syringyl/guaiacyl (S/G) constituent ratio] are two major barriers to wood-pulp production. To verify our contention that these traits are regulated by distinct monolignol biosynthesis genes, encoding 4-coumarate-CoA ligase (4CL) and coniferaldehyde 5-hydroxylase (CAld5H), we used Agrobacterium to cotransfer antisense 4CL and sense CAld5H genes into aspen (Populus tremuloides). Trees expressing each one and both of the transgenes were produced with high efficiency. Lignin reduction by as much as 40% with 14% cellulose augmentation was achieved in antisense 4CL plants; S/G-ratio increases as much as 3-fold were observed without lignin quantity change in sense CAld5H plants. Consistent with our contention, these effects were independent but additive, with plants expressing both transgenes having up to 52% less lignin, a 64% higher S/G ratio, and 30% more cellulose. An S/G-ratio increase also accelerated cell maturation in stem secondary xylem, pointing to a role for syringyl lignin moieties in coordinating xylem secondary wall biosynthesis. The results suggest that this multigene cotransfer system should be broadly useful for plant genetic engineering and functional genomics.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Laigeng</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>School of Forestry and Wood Products, Michigan Technological University, Houghton, MI 49931, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Yihua</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Xiaofei</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Jiayan</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Marita</LastName><ForeName>Jane M</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Ralph</LastName><ForeName>John</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Chiang</LastName><ForeName>Vincent L</ForeName><Initials>VL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2003</Year><Month>03</Month><Day>31</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance 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Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006899" MajorTopicYN="N">Mixed Function Oxygenases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008954" MajorTopicYN="N">Models, Biological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="Y">Plant Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName 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IdType="pubmed">15012247</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region></list><tree><noCountry><name sortKey="Cheng, Xiaofei" sort="Cheng, Xiaofei" uniqKey="Cheng X" first="Xiaofei" last="Cheng">Xiaofei Cheng</name><name sortKey="Chiang, Vincent L" sort="Chiang, Vincent L" uniqKey="Chiang V" first="Vincent L" last="Chiang">Vincent L. Chiang</name><name sortKey="Marita, Jane M" sort="Marita, Jane M" uniqKey="Marita J" first="Jane M" last="Marita">Jane M. Marita</name><name sortKey="Ralph, John" sort="Ralph, John" uniqKey="Ralph J" first="John" last="Ralph">John Ralph</name><name sortKey="Sun, Jiayan" sort="Sun, Jiayan" uniqKey="Sun J" first="Jiayan" last="Sun">Jiayan Sun</name><name sortKey="Zhou, Yihua" sort="Zhou, Yihua" uniqKey="Zhou Y" first="Yihua" last="Zhou">Yihua Zhou</name></noCountry><country name="États-Unis"><region name="Michigan"><name sortKey="Li, Laigeng" sort="Li, Laigeng" uniqKey="Li L" first="Laigeng" last="Li">Laigeng Li</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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